The Evidence

1. Naloxone blocked acupuncture analgesia
   
   
2. Six opiates antagonist block acupuncture analgesia
   
   
3. Dextro-naloxone doesn't block acupuncture analgesia
   
   
4. Antibodies to endorphins block acupuncture analgesia
   
   
5. Micro-injection of naloxone blocks acupuncture analgesia
   
   
6. Genetic defects in opiate receptors causes less acupuncture analgesia
   
   
7. Deficiency in endorphins causes less acupuncture analgesia
   
   
8. Endorphins rise in cerebral spinal fluid and drop in the brain after acupuncture analgesia
   
   
9. Acupuncture analgesia is enhanced by protecting from enzyme destruction
   
   
10. Cross circulation of acupuncture analgesic effects
    
    
11. Reduce pituitary endorphins block acupuncture analgesia
    
    
12. A rise in mRNA for proenkephalin with acupuncture analgesia
    
    
13. C-fos gene protein rises in endorphin areas of brain
    
    
14. Acupuncture analgesia shows cross tolerance with morphine addiction
    
    
15. Acupuncture analgesia works best for emotional pain like endorphin
    
    
16. Lesions of arcuate nucleus blocks acupuncture analgesia
    
    
17. Lesions of periaquaductal gray blocks acupuncture analgesia

The considerable evidence for the three pathways shown above provides convincing proof that acupuncture analgesia is a known physiological phenomenon that can occur and be manipulated through the use of acupuncture needles and electrical stimulation.

Testing the involvement of the pituitary, several experiments were carried out, both surgically removable pituitary and suppression of the pituitary endorphins by chemical manipulations, all of the experiments suppressed acupuncture analgesia in animals. Experiments to test the involvement of the mid brain was done since it has been shown that morphine pain relief was largely mediated by this system. Such experiments include direct lesions to the raphe by cutting the out put fibers in the dorsal lateral tract, the spinal cord blockade of serotonin receptors, blockade of serotonin synthesis and direct micro-injection of naloxone into the mid brain; all of these experiments reduced acupuncture analgesia.

Enhancement of serotonin synthesis increased acupuncture analgesia. An experiment measuring serotonin showed an increase product (serotonin) was released during acupuncture analgesia along with noradrenelinecture endorphin mechanisms verifying and supporting acupuncture analgesia.